Curcumin Enhanced Busulfan-Induced Apoptosis through Downregulating the Expression of Survivin in Leukemia Stem-Like KG1a Cells
Table 1
Expression of apoptosis-related proteins in various treated groups.
Name
Control
CUR
BUS
CUR + BUS
CUR/control
BUS/control
CUR + BUS/control
(fold-change)
(fold-change)
(fold-change)
BAD
9754.372
11939.256
8854.383
21865.952
1.224
0.908
2.242
BAX
11665.057
11534.453
11072.484
10875.680
0.989
0.949
0.932
Bcl-2
5201.059
4032.165
4793.070
2454.999
0.775
0.922
0.472
Bcl-w
3934.754
3354.182
3464.131
3789.188
0.852
0.880
0.963
BID
1241.608
1159.421
1473.127
1702.301
0.934
1.186
1.371
BIM
8791.171
7763.556
8225.960
8295.705
0.883
0.936
0.944
Caspase-3
5414.356
9883.863
4966.096
12514.137
1.825
0.917
2.311
Caspase-8
6636.880
7681.166
7505.017
7338.462
1.157
1.131
1.106
CD40
7229.618
5707.767
6661.514
5874.868
0.789
0.921
0.813
CD40L
16087.024
12956.135
15884.779
14392.403
0.805
0.987
0.895
cIAP-2
1971.306
1308.320
1917.709
702.301
0.664
0.973
0.356
CytoC
8302.835
6758.989
8156.269
7832.756
0.814
0.982
0.943
DR6
3370.080
2669.250
3008.736
2958.532
0.792
0.893
0.878
Fas
25854.867
22694.082
24157.601
23693.574
0.878
0.934
0.916
FasL
7155.526
6020.454
7420.907
7047.913
0.841
1.037
0.985
HSP27
2395.653
2109.392
2279.382
2807.833
0.881
0.951
1.172
HSP60
24408.943
20936.090
30040.496
23881.647
0.858
1.231
0.978
HSP70
6055.367
7532.267
6146.040
6971.961
1.244
1.015
1.151
HTRA
10052.830
20991.713
12382.184
26027.247
2.088
1.232
2.589
IGF-I
1772.604
1407.585
1857.630
1717.974
0.794
1.048
0.969
IGF-II
7991.872
8066.316
10003.085
9495.273
1.009
1.252
1.188
IGFBP-1
3060.239
2144.135
2680.707
2416.014
0.701
0.876
0.789
IGFBP-2
3750.645
2977.966
3901.504
3569.769
0.794
1.040
0.952
IGFBP-3
5321.179
3985.511
5547.657
5313.061
0.749
1.043
0.998
IGFBP-4
2012.843
1573.358
1736.271
1873.496
0.782
0.863
0.931
IGFBP-5
11366.442
9202.906
10929.497
10428.404
0.810
0.962
0.917
IGFBP-6
2354.116
1950.567
2340.662
2413.603
0.829
0.994
1.025
IGF-1sR
5755.629
4286.285
5791.576
5056.269
0.745
1.006
0.878
Livin
7478.838
6829.468
7818.628
7778.504
0.913
1.045
1.040
p21
17207.390
15850.718
18463.352
17993.517
0.921
1.073
1.046
p27
8486.943
7790.358
8890.430
8879.213
0.918
1.048
1.046
p53
9829.587
9303.164
11354.853
11263.882
0.946
1.155
1.146
SMAC
9838.568
10157.840
11915.987
12549.047
1.032
1.211
1.275
Survivin
76507.100
31134.629
81877.505
11691.497
0.407
1.070
0.153
sTNF-R1
3284.761
2747.670
3781.346
3136.960
0.836
1.151
0.955
sTNF-R2
3504.793
2428.035
3118.079
2815.066
0.693
0.890
0.803
TNF-alpha
2641.505
1771.889
2802.065
2499.200
0.671
1.061
0.946
TNF-beta
6946.720
4871.952
6715.585
6430.648
0.701
0.967
0.926
TRAILR-1
3835.964
3031.569
4114.182
4102.643
0.790
1.073
1.070
TRAILR-2
7488.942
6315.272
7505.017
7691.701
0.843
1.002
1.027
TRAILR-3
4649.857
3717.494
4614.036
4605.376
0.799
0.992
0.990
TRAILR-4
4613.933
3900.142
4694.541
4706.646
0.845
1.017
1.020
XIAP
5465.996
2412.352
6570.195
1011.597
0.441
1.202
0.185
KG1a cells were treated with CUR (16 μM), BUS (80 μM) alone, or CUR + BUS for 48 h tested by protein arrays kit. The data represent fluorescence intensities of 43 apoptosis-related proteins. The bold bands indicate proteins that were modulated by CUR or CUR + BUS. The threshold values of fold-change were usually set at ≤0.667 or ≥1.5.
