Behaviour of Endothelial Cells in a Tridimensional In Vitro Environment

Figure 2

Endothelial cell purity and effect of conditioned medium from monolayer cultures. (a) Flow cytometry results of HDMECs. (b) CD31-Alexa 488 (green) IF staining of monolayer HDMECs (20x magnification, scale bar = 100 μm). Effect of conditioned media (CM) on endothelial cell monolayer culture organization and physiology on four-day-old culture. (c) Monolayer keratinocyte conditioned medium (KCM); circular branch-like structures were indicated with blue arrows. (d) Monolayer fibroblast conditioned medium (FCM). (e) Mixture of fibroblast and keratinocyte conditioned medium (MFKCM, 1 : 1). (f) EGM-2 MV SingleQuots Kit media with supplements and growth factors (EBM-2). (g) HDMECs proliferation assay with CM.