Behaviour of Endothelial Cells in a Tridimensional In Vitro Environment

Figure 4

Observation of endothelial cell behaviour with whole-mount immunostaining. The steps of capillary formation in the in vitro skin model on a weekly basis after EC seeding: (a, b) fifth week, (c, d) sixth week, (e) seventh week, (f) eighth week, (g) lumen formation (40x magnification), and (h, i) ninth week, the end of the procedure (all figures (except (g)) were captured at 20x magnification with confocal microscopy) ((scale bar = 100 μm for (a), (b), (c), (e), (h), and (i)) – (scale bar = 50 μm for (f) and (g)) and scale bar = 200 μm for (d)).