Behaviour of Endothelial Cells in a Tridimensional In Vitro Environment

Figure 5

Biochemical analysis with the conditioned media of substitutes. ELISA ( or 6), MMP activity (), and western blot results. ELISA results of (a) VEGF and (b) Ang-1. VEGF staining with IHC of (c) bilayered skin model and (d) ex vivo human skin (10x magnification, scale bar = 100 μm). (e) ELISA results of IL-8. (f) MMP activity analysis of bilayered and dermal substitutes and (g) collagen-I deposition of substitutes.

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