Role of TLR4-Mediated PI3K/AKT/GSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes

Figure 6

TLR4/PI3K/AKT/GSK-3β signaling pathway inhibition affects production of Bax, Bcl-2, and active caspase-3. The five groups were treated as above 4. (a) The mRNA expression of Bax/Bcl-2 and caspase-3 was detected by RT-qPCR. (b) Quantification (relative density) of the intensity of staining of active caspase-3, Bax, and Bcl-2 protein detected by Western blot. (c) Representative Western blot of active caspase-3, Bax, and Bcl-2 protein from BRL-3A cells. β-actin was used as an internal control. Data were mean ± SEM (). versus control group. versus LPS group.

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