The Roles of miR-26, miR-29, and miR-203 in the Silencing of the Epigenetic Machinery during Melanocyte Transformation

Figure 1

Metastatic melanoma cells are hypomethylated compared with nontumorigenic melan-a cells. The methylation status of three specific CpGs associated with A-repeats (a) and two CpGs in the retrovirus-C repetitive sequence (b) were evaluated using Ms-SNuPE in the melan-a, 4C3−, and 4C3+ cell lines. The percentage of methylation was determined by calculating the average at the different CpG sites analyzed. Ma: nontumorigenic melan-a melanocyte line; 4C3−: nonmetastatic melanoma cell line; 4C3+: metastatic melanoma cell line. .

(a)

(b)