Enhanced and Secretory Expression of Human Granulocyte Colony Stimulating Factor by Bacillus subtilis SCK6

Figure 3

(a) Purification of recombinant human GCSF by FPLC on QFF column. Inset shows the purified GCSF eluted with 0.3 M NaCl concentration gradient. Blue and red colors show absorbance at and , respectively. (b) CD spectrum of the recombinant in-house produced GCSF (solid line) and the commercially available GCSF preparation, that is, Filgrastim (dotted line), scanned over 185–260 nm range.

(a)

(b)