Enhanced and Secretory Expression of Human Granulocyte Colony Stimulating Factor by Bacillus subtilis SCK6

Figure 4

(a) GCSF biological activity assay. Left, mice being injected with GCSF by subcutaneous route; right, microscopic analysis of Giemsa stained slides wherein the encircled cells represent the neutrophil counts. (b) Mice in the sample and the control group received two different doses of GCSF (15 and 40 μg/mL/mouse). The control group was treated with 0.1% BSA in PBS. The abbreviations cGCSF and rhGCSF stand for commercially available GCSF and in-house produced recombinant human GCSF, respectively.

(a)

(b)