Zebrafish as a Model for the Study of Human Myeloid Malignancies

Table 1

Zebrafish animal models of myeloid malignancies.

Gene name	Promoter and expression construct	Type	Refs
MYCN	MYCN-HSE-EGFP	AML	[58]
MYST3/NCOA2	spi-1-MYST3/NCOA2-EGFP	AML	[70]
kRASG12D	B-actin-LoxP-EGFP-LoxP-kRASG12D; hsp70-Cre	MPD	[61]
NUP98-HOXA9	spi-1-loxP-EGFP-loxP-NUP98-HOXA9; hsp70-Cre	MPN	[62]
Stat5.1 mutants	Constitutively active mutants of Stat5.1	Tumor-like lesions; increased numbers of early and late myeloid cells, erythrocytes, and B cells	[65]
NPM1 mutant	pCS2cmv-NPM1c-EGFP	No AML; increased numbers of definitive hematopoietic cells	[66]
tel-jak2a	pCS2cmv-Flag-tel-jak2a; spi-1-Flag-tel-jak2a	No AML; anemic; perturbed intermediate cell mass; accumulation of large cells near the heart	[67]
AML1	pCS2cmv-runx1	No AML; enlarged heart and ectopic blood	[24]
AML1-ETO	pCS2cmv-RUNX1-CBF2T1	No AML; defective development of blood and circulation and internal hemorrhaging	[24]
AML1-ETO	hsp-AML1-ETO	No AML; loss of gata1 hematopoietic cells in the posterior blood islands	[68]
AML1-ETO + Gro3 MO	hsp-AML1-ETO	No apparent AML; enhanced the accumulation of blast cells	[68]
FLT3-ITD	CMV-FLT3-ITD-T2a-EGFP	Ectopic myeloid cell expansion
	CMV-FLT3-TKD-T2a-EGFP	Ectopic myeloid cell expansion resistant to AC220	[69]
	CMV-FLT3-ITD-TKD-T2a-EGFP	Conferred resistance to AC220 treatment

AML: acute myeloid leukemia; MPD: myeloproliferative disorder; MPN: myeloproliferative neoplasm; MO: morpholino.