Sensitive Detection of Thirteen Bacterial Vaginosis-Associated Agents Using Multiplex Polymerase Chain Reaction

Table 2

M-PCR validation based compared to sPCR for thirteen major BV-AAs in cervical-vaginal samples.

Agents	Sensibility (%)	Specificity (%)	PPV (%)	NPV (%)	Accuracy (%)
Overall	99.1	100.0	100.0	97.0	99.3
Mobiluncus curtisii	100.0	100.0	100.0	100.0	100.0
Ureaplasma urealyticum	100.0	100.0	100.0	100.0	100.0
Mycoplasma hominis	80.0	100.0	100.0	97.8	98.0
Gardnerella vaginalis	100.0	100.0	100.0	100.0	100.0
Megasphaera type 1	100.0	100.0	100.0	100.0	100.0
Bacteroides fragilis	100.0	100.0	100.0	100.0	100.0
Atopobium vaginae	100.0	100.0	100.0	100.0	100.0
BVAB1	100.0	100.0	100.0	100.0	100.0
BVAB2	88.8	100.0	100.0	97.6	98.0
BVAB3	100.0	100.0	100.0	100.0	100.0
Mycoplasma genitalium	100.0	100.0	100.0	100.0	100.0
Mobiluncus mulieris	100.0	100.0	100.0	100.0	100.0
Sneathia sanguinegens	100.0	100.0	100.0	100.0	100.0
Two or more agents simultaneously	100.0	100.0	100.0	100.0	100.0

M-PCR: multiplex polymerase chain reaction; sPCR: single polymerase chain reaction; BV-AAs: bacterial vaginosis-associated agents; PPV: positive predictive value; NPV: negative predictive value.