Krill Oil Ameliorates Mitochondrial Dysfunctions in Rats Treated with High-Fat Diet

Figure 3

Effect of KO on respiratory complexes activity, expression, and function. Effect of KO on respiratory complexes activity (a). Enzymatic activities measured in the control group were set to 100%. Effect of KO on OXPHOS proteins levels (b). Liver mitochondrial proteins from control, HF, or HF+KO-fed rats were separated by SDS-PAGE, transferred to nitrocellulose, and then immunodecorated with a cocktail against NDUFB8, SDHB, COR2, COX1, ATP5A, or the mammalian porin as a control. The amount of OXPHOS proteins revealed by immunodecoration in the control group was set to 100% (c). ATP and LPO quantification of liver samples isolated from animals fed a control diet, a HF diet, or a HF+KO (d). The amount of ATP or LPO (expressed as nmoles per mg protein) in the control group was set to 100%. The values reported in the figure represent the means ± SD (). versus rats fed control diet; versus rats fed HF diet.

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