Triphala Extract Suppresses Proliferation and Induces Apoptosis in Human Colon Cancer Stem Cells via Suppressing c-Myc/Cyclin D1 and Elevation of Bax/Bcl-2 Ratio

Figure 1

Total phenolics, antioxidant activity, and antiproliferative efficacy of serial solvent extracts of triphala. Total phenolics and antioxidant activity (a) were determined by Folin-Ciocalteu reagent and ABTS assay, respectively, as described in Section 2. Antiproliferative efficacy (b) was determined using BrdU assay by treating the HCCSCs with solvent control: DMSO, FU: 5-fluorouracil (25 and 50 μg/mL), and serial solvent extracts of triphala at 25, 50, or 100 μg/mL for 20 hours. Results were expressed as mean ± SE for three experiments at each time point. GAE: gallic acid equivalents; TE: Trolox equivalents. The error bars with the same letter indicate no significance at .

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