Triphala Extract Suppresses Proliferation and Induces Apoptosis in Human Colon Cancer Stem Cells via Suppressing c-Myc/Cyclin D1 and Elevation of Bax/Bcl-2 Ratio

Figure 2

Total phenolics, antioxidant activity, and antiproliferative efficacy of direct solvent extractions of triphala. Total phenolics and antioxidant potential (a) were determined by Folin-Ciocalteu reagent and ABTS assay, respectively. Antiproliferative efficacy (b) was determined using BrdU assay by treating HCCSCs with solvent control: DMSO, FU: 5-fluorouracil (25 or 50 μg/mL), and solvent extracts of triphala at 25, 50, or 100 μg/mL for 20 hours. AET: acetone extract of triphala, MET: methanol extract of triphala, EET: ethanol extract of triphala, and WET: water extract of triphala. Results were expressed as mean ± SE for three experiments at each time point. GAE: gallic acid equivalents; TE: Trolox equivalents. The error bars with the same letter indicate no significance at .

(a)

(b)