Figure 4: Effect of methanol extract of triphala (MET) on apoptosis of HCCSCs (a) and HCCSC p53-shRNA cells (b). Cells were treated with IC(50) of 5-fluorouracil (75 μg/mL) and MET (104 μg/mL) and apoptosis was analyzed by western blot. Data are representative of three individual experiments; a representative blot is presented. M: media control, S: solvent control, FU: 5-fluorouracil, and MET: methanol extract of triphala. The error bars with the same letter indicate no significance at .