Triphala Extract Suppresses Proliferation and Induces Apoptosis in Human Colon Cancer Stem Cells via Suppressing c-Myc/Cyclin D1 and Elevation of Bax/Bcl-2 Ratio

Table 1

Tentative compounds determined by LC-ESI-QTOF-MS-MS in methanol extract of triphala (MET).

No.		RT^a	Score^b	Tentative identified molecule^c	MassBank ID	Molecular formula	Exact mass	Area under; curve
1	617.50	15.66	0.853	Hypaconitine	TY000048	C33H45NO10	615.30	24347
2	345.05	0.52	0.805	Naringin	PB000804	C27H32O14	580.18	139768
3	607.21	5.99	0.800	Embinin	TY000131	C29H34O14	606.19	6610
4	135.95	19.94	0.760	Pseudopelletierine	KOX00830	C9H15NO	153.12	560
5	687.14	1.38	0.750	Xanthohumol	CE000112	C21H22O5	354.15	9876
6	186.96	19.49	0.743	Atomoxetine	EA284610	C17H21NO	255.16	363
7	593.27	12.13	0.737	Acaciin	PR020027	C28H32O14	592.18	19729
8	493.25	6.77	0.695	Malvidin-3-O-beta-D-galactoside	PR020065	C23H25O12	493.13	4302
9	449.07	4.59	0.686	Isorhamnetin	TY000220	C16H12O7	316.06	33552
10	471.02	3.01	0.681	Homoorientin	PR040134	C21H20O11	448.10	75557
11	364.02	2.06	0.680	2-{2-Benzimidazol-2-yl-1-[(4-methylphenyl)methyl]; ethyl}benzimidazole	BML80240	C24H22N4	366.18	330
12	609.27	11.61	0.674	Reserpine	CE000152	C33H40N2O9	608.27	429
13	97.97	19.71	0.672	Sotalol	EA017004	C12H20N2O3S	272.12	1287
14	130.01	0.08	0.671	Epoxiconazole	EA009507	C17H13ClFN3O	329.07	722
15	677.38	9.35	0.670	Icariin	TY000037	C33H40O15	676.24	2828
16	409.16	7.46	0.670	Nodakenin	TY000089	C20H24O9	408.14	6173
17	303.05	4.63	0.660	Quercetin	PB006206	C15H10O7	302.04	82528
18	557.09	13.91	0.640	Fukugetin	TY000181	C30H20O11	556.10	498
19	411.36	10.65	0.616	Geniposide	TY000059	C17H24O10	388.14	3366

The retention times (RT) (in minutes) are according to the LC-MS system. ^bSimilarity score of retrieved spectrum to a query data from the MassBank database. ^cTentative molecules were identified based on high score [33]. Confirmed using comparison on retention time and mass spectra with analytical standard naringin.