Discrepancies in Drug Susceptibility Test for Tuberculosis Patients Resulted from the Mixed Infection and the Testing System

Table 1

Primers used for PCR amplification in this study.

Gene	Forward primer, 5′-3′	Reverse primer, 5′-3′
katG	GCT GCT GTG GCC GGT CAA GA	CGT CCT TGG CGG TGT ATT GC
inhA reg	CCT CGC TGC CCA GAA AGG GA	ATC CCC CGG TTT CCT CCG GT
inhA ORF	GAA CTC GAC GTG CAA AAC	CAT CGA AGC ATA CGA ATA
oxyR-ahpC	CTG CGA CGG TGC TGG CACG	CAC GCT GCT GCG GGT GAT TGA T
MIRU and spoligotyping cluster for M. tuberculosis isolates
Spoligotyping	GGT TTT GGG TCT GAC GAC	CCG AGA GGG GAC GGA AAC
MIRU02	TGG ACT TGC AGC AAT GGA CCA ACT	TAC TCG GAC GCC GGC TCA AAA T
MIRU04	GCG CGA GAG CCC GAA CTG C	GCG CAG CAG AAA CGT CAG C
MIRU10	GTT CTT GAC CAA CTG CAG TCG TCC	GCC ACC TTG GTG ATC AGC TAC CT
MIRU16	TCG GAG AGA TGC CCT TCG AGT TAG	CCC GTC GTG CAG CCC TGG TAC
MIRU20	TCG GAG AGA TGC CCT TCG AGT TAG	GGA GAC CGC GAC CAG GTA CTT GTA
MIRU23	CTG TCG ATG GCC GCA ACA AAA CG	AGC TCA ACG GGT TCG CCC TTT TGT C
MIRU24	CGA CCA AGA TGT GCA GGA ATA CAT	GGG CGA GTT GAG CTC ACA GAA
MIRU26	TAG GTC TAC CGT CGA AAT CTG TGA C	CAT AGG CGA CCA GGC GAA TAG
MIRU27	TCG AAA GCC TCT GCG TGC CAG TAA	GCG ATG TGA GCG TGC CAC TCA A
MIRU31	ACT GAT TGG CTT CAT ACG GCT TTA	GTG CCG ACG TGG TCT TGA T
MIRU39	CGC ATC GAC AAA CTG GAG CCA AAC	CGG AAA CGT CTA CGC CCC ACA CAT
MIRU40	GGG TTG CTG GAT GAC AAC GTG T	GGG TGA TCT CGG CGA AAT CAG ATA