The Type III Secretion System (T3SS) of Chlamydophila psittaci Is Involved in the Host Inflammatory Response by Activating the JNK/ERK Signaling Pathway

Figure 1

Inclusion-forming units (IFUs) of C. psittaci in THP-1 cells treated with iron sulfate, INP0007, or both. (a) Number of IFUs in each group. One million THP-1 cells were treated with 50 μM FeSO₄, 50 μM INP0007, or both; the cells were then infected with C. psittaci. At 44 h p.i., the THP-1 cells were fixed and incubated with a rabbit anti-C. psittaci 6BC antibody and then incubated with a Cy2-labeled (green fluorescence) goat anti-rabbit IgG and DAPI. The inclusion bodies (green fluorescence) in the cells were counted under a fluorescence microscope (at 40x magnification). Five fields of each sample were counted. (b) Images of IFUs of C. psittaci in THP-1 cells. Cells infected with C. psittaci (A), cells infected with C. psittaci and pretreated with INP0007 (B), cells infected with C. psittaci and pretreated with FeSO₄ (C), and cells infected with C. psittaci and pretreated with both INP0007 and FeSO₄ (D) (40x magnification).

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