BioMed Research International / 2015 / Article / Fig 2

Research Article

The Type III Secretion System (T3SS) of Chlamydophila psittaci Is Involved in the Host Inflammatory Response by Activating the JNK/ERK Signaling Pathway

Figure 2

Production of IL-8, IL-6, TNF-α, and IL-1β in C. psittaci-infected THP-1 cells that were pretreated with iron sulfate (50 μM FeSO4), 50 μM INP0007, or both. THP-1 cells were infected with C. psittaci in the presence of 50 μM INP0007 and/or 50 μM FeSO4. Mock group: THP-1 cells were treated with heat inactivated Cps. At 44 h p.i., the levels of IL-8 (a), IL-6 (c), TNF-α (e), and IL-β (g) in the supernatants were assayed by ELISA. Total RNA was isolated from the THP-1 cells, and the transcription levels of IL-8 (b), IL-6 (d), TNF-α (f), or IL-β (h) were analyzed by Q-PCR. Cps, THP-1 cells infected with C. psittaci. Cps-Fe, THP-1 cells infected with C. psittaci and pretreated with FeSO4. Cps-INP, THP-1 cells infected with C. psittaci and pretreated with INP0007. Cps-Fe-INP, THP-1 cells infected with C. psittaci and pretreated with both FeSO4 and INP0007. compared with the Cps group.
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