Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

Figure 2

Deguelin induced cell death in SCC-4 and HSC-4 cell lines. Phase-contrast microscopic analysis. SCC-4 cells were treated with 0 or 100 M deguelin in DMEM + 10% FBS. After 24 h incubation, photographs were taken under phase-contrast microscopy. Representative phase-contrast micrographs are shown (a). Bar: 50 m. Trypan-blue dye exclusion assay was performed to measure cell viability of SCC-4 cells (b) and HSC-4 cells (c) at 24 h after 100 M deguelin treatment. Arrows indicate initial cell numbers. Each point represents the mean ± SD from triplicate assay ().

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