Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

Figure 3

Deguelin induced apoptosis in SCC-4 cell lines. SCC-4 cells were incubated in the absence or presence of 100 M deguelin for different times. Thereafter, the cells were washed and fixed. They were further stained with propidium iodide (PI, -axis) to detect accumulation of cell cycle phase (a) and treated with anti-annexin V antibody conjugated with FITC (FITC, -axis) to analyze apoptosis (b) by flow cytometry.

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(b)

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