Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

Figure 4

Deguelin reduced the expression of phosphorylated IGF1R, p-Akt, and p-ERK and induced apoptosis in SCC-4 cell lines. Subconfluent culture was treated with deguelin at different concentrations for 24 h. Whole-cell extracts were prepared and analyzed by Western blot using antibodies against p-Akt, Akt, p-ERK, and ERK (a); p-EGFR, EGFR, p-IGF1R, and IGF1R (b); and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP) (c). Total cell extracts from Jurkat cells: serum starved overnight and then treated with Calyculin A was used as positive control (PC) for p-Akt and Akt.

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