Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

Figure 6

Deguelin induced apoptosis by targeting both EGFR-Akt and IGF1R-Akt pathways in HNSCC cell lines. Subconfluent cultures were incubated for 24 h in serum-free medium. After the starvation, cells were treated with 10 M deguelin for 1 h. (a) The deguelin-treated SCC-4 cells were incubated for 15 min and 24 h with or without 10 ng/ml of IGF, respectively. (b) The deguelin-treated HSC-4 cells were incubated for 24 h with or without 10 ng/ml of EGF. Whole-cell extracts were analyzed by Western blot using antibodies against p-Akt, Akt, and PARP. (c) HSC-4 cells were treated with deguelin at different concentrations for 24 h in 10% FBS-containing medium. Whole-cell extracts were analyzed by Western blot using antibodies against p-EGFR, EGFR, and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP).

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