Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro

Figure 3

Characterization of porcine DFAT cells during the long-term culture. (a) Different passages of DFAT cells at P10, P20, P30, P40, P50, and P60 uniformly displayed fibroblast-like morphology, bar, 50 μm. (b) Quantification of viability of P5, P29, and P59 DFAT cells by trypan blue staining. (c) Population doubling time (PDT) of DFAT cells at P10, P30, and P60 was  h,  h, and  h, respectively. (d) Cumulative population doubling (CPD) detected from P1 to P16 reached in 58-day culture. (e) The karyotype analysis of P8, P30, and P60 DFAT cells with Giemsa staining. Values are the mean ± SD of triplicate dishes. NS means no significant difference ().

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