A New Mechanism of Vitamin C Effects on A/FM/1/47(H1N1) Virus-Induced Pneumonia in Restraint-Stressed Mice

Figure 4

Effects of vitamin C on gene and protein expressions of proinflammatory cytokines in lung tissue of restraint-stressed mice after infection. (a) IL-1β, IL-6 mRNA expression in lung tissue from 10 mice in each group were determined by RT-PCR and normalized by 18S. (b) The protein levels of TNF-α in lung tissue were determined by western blotting and normalized by β-actin. N, normal; V, virus; M, model; R, ribavirin; E, edaravone; Vc-L, vitamin C-low dosage; Vc-H, vitamin C-high dosage. Significant differences from the normal group at , from the virus group at , , and from the model group at , .

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