BioMed Research International / 2015 / Article / Tab 3

Research Article

A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

Table 3

Comparison of the earliest antibody detection using flavivirus ELISA, MIA, and VNT. Identification of specific WNV, JEV, or TBEV antibodies in reference sera sampled on days 0, 4, 8, 11, 14, 20, 35, and 58.

ELISA positive(1)MIAMNT
Positive for WNV.sE(2)Flavivirus
identification(3)
Positive(4)Flavivirus identification(5)

SeraWNV
lineage 1
D11D11D35D8D14
WNV
lineage 2
D11D8D8D8D14
JEV
TBEV
D20
D20
D20
D20
Not achieved
D35
D20
D8
D35
D8

(1)ID Screen West Nile competition ELISA kit (ID Vet). Sample positive if %S/N 40%, doubtful if 40 %S/N 50%, and negative if %S/N 50.
(2)WNV.sE positive if S/P ratio 17 for the WNV.sE bead.
(3)WNV.EDIII identification if S/P ratio 17 for the WNV.sE bead and S/P ratio > 54 for the WNV.EDIII bead.
JEV.EDIII identification if S/P ratio > 17 for the WNV.sE bead and S/P ratio > 55 for the JEV.EDIII bead; TBEV.EDIII identification if S/P ratio 61 for the TBEV.EDIII bead.
(4)MNT positive if MNT titre threshold (10 for JEV and WNV and 20 for TBEV).
(5)MNT identification of the flavivirus with the highest neutralisation capacity and at least a fourfold difference in titres.