CD40 Ligand Deficient C57BL/6 Mouse Is a Potential Surrogate Model of Human X-Linked Hyper IgM (X-HIGM) Syndrome for Characterizing Immune Responses against Pathogens
Morphological and histological analysis. Colons of wild type (WT) and C57-CD40L−/− mice uninfected and orally inoculated with CFU of C. rodentium were removed at day 14 after inoculation (p.i.). (a) Colon length. Infected C57-CD40L−/− mice colons lengths were significantly shorter than colons of infected WT mice. (b) Colon weight. Colons of infected-WT and -C57-CD40L−/− mice weighted more compared with colons of uninfected mice. (c) Villi high. Intestinal villi of both infected mice strains were significantly higher than villi of uninfected mice. Villi of infected WT mice were higher compared with villi of infected C57-CD40L−/− mice. Mean comparisons were done by paired Student’s -test. (d) Hematoxylin and eosin stained sections of distal colons. Arrows denote submucosal inflammatory cellular infiltrates and epithelial damage in the mucosa of infected mice.