Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification

Figure 1

Vector constructs for GCR and GC proteins and functional assay of the GCR and GC proteins. (a) Schematic representation of plasmid vectors expressing GCR or GC proteins. The vector was designed to produce a fusion protein consisting of Gluc, mCherry, and the RGD motif, or Gluc and mCherry driven by the T7 promoter. (b) The luciferase activity of the medium and the cell lysates was measured for verification of successul production of the fusion proteins by the expression vectors. The luciferase activity of the growth medium was higher than that of the bacterial lysate (). GCR, sGluc-mCherry-RGDX3; GC, sGluc-mCherry; sGluc, secretory Gaussia luciferase; RGD, Arg-Gly-Asp.

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