Advanced Glycation End Products Induce Endothelial-to-Mesenchymal Transition via Downregulating Sirt 1 and Upregulating TGF-β in Human Endothelial Cells

Figure 2

EndMT-associated molecules were regulated by AGE-BSA in protein level. (a) Western blot assay of CD 31, FSP-1, α-SMA, collagen I, and β-actin in HUVECs which were treated with 50, 100, or 300 μg/mL AGE-BSA or BSA for 96 h. ((b)–(e)) Relative level of CD 31 (b), FSP-1 (c), α-SMA (d), and collagen I (e) to β-actin in HUVECs which were treated with AGE-BSA or BSA with various concentrations. Each value was averaged for triple independent experiments; statistical significance was shown as , ; ns: no significance.

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