Advanced Glycation End Products Induce Endothelial-to-Mesenchymal Transition via Downregulating Sirt 1 and Upregulating TGF-β in Human Endothelial Cells

Figure 4

Sirt 1 was promoted by AGE-BSA in HUVECs. ((a) and (b)) Relative mRNA level of Sirt 1 (a) or Sirt 2 (b) to β-actin in HUVECs which were treated with 50, 100, or 300 μg/mL AGE-BSA or BSA for 48 h. (c) Western blot analysis of Sirt 1 and Sirt 2 in protein level in AGE-BSA- or BSA-treated HUVECs. (d) Relative Sirt 1 activity in HUVECs which were treated with 50, 100, and 300 μg/mL AGE-BSA or 300 μg/mL BSA for 96 h. All experiments were performed in triplicate, and statistical significance was considered when or less, , , and ; ns: no significance.

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