Advanced Glycation End Products Induce Endothelial-to-Mesenchymal Transition via Downregulating Sirt 1 and Upregulating TGF-β in Human Endothelial Cells

Figure 5

Chemical manipulation of Sirt 1 activity regulated the AGE-BSA-induced, TGF-β-mediated EndMT in HUVECs. (a) Regulation of Sirt 1 activity in the AGE-BSA-treated HUVECs by RSV (2 or 10 μM) or by EX527 (0.1 or 0.5 μM) for 48 h. (b) Sirt 1 mRNA level in HUVECs treated with 100 μg/mL AGE-BSA and Sirt 1 regulators for 48 h. (c) Western blot analysis of TGF-β, CD 31, and FSP-1 in AGE-BSA-treated HUVECs, after Sirt 1 activity for 96 h. (d) Inhibition by RSV or aggravation by EX527 on TGF-β induction by AGE-BSA in HUVECs for 96 h. (e) Regulation by RSV or EX527 on the CD 31/FSP-1 in AGE-BSA-treated HUVECs. All results were averaged for three independent experiments. , , and ; ns: no significance.

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