BioMed Research International / 2015 / Article / Fig 2

Research Article

Zeaxanthin Inhibits Hypoxia-Induced VEGF Secretion by RPE Cells through Decreased Protein Levels of Hypoxia-Inducible Factors-1α

Figure 2

Effects of hypoxia on secretion of VEGF by RPE cells. RPE cells were seeded into 24-well plates at a density of cells per well. After 24 h, cells were incubated in a sealed chamber in a controlled environment of 1% O2 (a) or cultured with CoCl2 at 50, 100, 150, and 200μM concentrations (b). Cells cultured under normal oxygen pressure (21% O2, 5% CO2, and 74% N2) were served as the control. Conditioned medium was collected 24 h later; VEGF levels were measured by VEGF ELISA kits and expressed as percentages of the control (, ). Hypoxia significantly increased VEGF secretion by RPE cells. , compared with the controls.

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