Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

Figure 1

Map of the T9W-IVSI-6 vector showing the XcmI and ClaI restriction sites, used to cut the vector for microinjection. The human β-globin genomic region containing the β⁺IVSI-6 thalassemic point mutation (grey box) is also reported. The final portion of the β-globin exon 1 and the first portion of the β-globin exon 2 are underlined and marked in bold characters. The position of mutagenesis primers is boxed with a dashed line.