Research Article
Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation
Table 1
PCR primers employed for identification and characterization of transgenic mice.
| Name | Sequence | Length (nt) | Melting temperature (°C) | Gene |
| MuActF | 5′ TGACGGGGTCAACCACACTGTGCCCATCTA 3′ | 30 | 81 | Murine β-actin | MuActR | 5′ CTAGAAGCATTTGCGGTGGACGATGGAGGG 3′ | 30 | 80 | Murine β-actin | TransF | 5′ TGCATTCATTTGTTGTTGTTTTTCT 3′ | 25 | 65 | Transgene (LCR) | TransF [6FAM] | 5′ [6-FAM] TGCATTCATTTGTTGTTGTTTTTCT 3′ | 25 | 65 | Transgene (LCR) | TransR | 5′ TGACTAAAACTCCACCTCAAACGG 3′ | 24 | 67 | Transgene (LCR) | HuBetaF | 5′ AGACCTCACCCTGTGGAGCC 3′ | 20 | 68 | Human β-globin | HuBetaR | 5′ TCAGGAGTGGACAGATCCCC 3′ | 20 | 67 | Human β-globin | MuActF1 [6FAM] | 5′ [6-FAM] TACTTTGGGAGTGGCAAGCC 3′ | 20 | 66 | Murine β-actin | MuActR1 | 5′ TCTCCATGTCGTCCCAGTTG 3′ | 20 | 66 | Murine β-actin |
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