Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

Table 2

Primers employed for RT-PCR analyses.

Name	Sequence	Length (nt)	Melting temperature (°C)	Transcript
HuBetaF1	5′ GCATCTGACTCCTGAGGAGAAGTC 3′	24	67	Human β-globin
HuBetaF1; [6FAM]	5′ [6-FAM] GCATCTGACTCCTGAGGAGAAGTC 3′	24	67	Human β-globin
HuBetaR	5′ TCAGGAGTGGACAGATCCCC 3′	20	67	Human β-globin
MuBetaF	5′ CCTGACTGATGCTGAGAAGGC 3′	21	66	Murine β-globin
MuBetaR	5′ GCAGAGGATAGGTCTCCAAAGCTATC 3′	26	67	Murine β-globin
MuAlphaF	5′ CTGAAGCCCTGGAAAGGATGT 3′	21	66	Murine α-globin
MuAlphaR	5′ ATTTGTCCAGAGAGGCATGCA 3′	21	67	Murine α-globin
MuActF2	5′ TGTATTCCCCTCCATCGTGG 3′	20	67	Murine β-actin
MuActR2	5′ CACAGCCTGGATGGCTACGTAC 3′	22	68	Murine β-actin
IVSI+13F	5′ GGGCAGGTTGGCATCAAG 3′	18	67	IVSI+13 altered spliced transcripts