Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

Table 3

Hematological data of transgenic mice carrying the human ^huβ^IVSI-6 globin locus.

Age (weeks)	Sex	Genotype	Hb (g/dL)	RBC (10^6/µL)	HCT (%)	MCV (fL)	MCH (pg)	MCHC (g/dL)	RDW (%)
16	♀	Wild-type ()	13.7 ± 0.4	8.2 ± 0.4	42.9 ± 1.8	52.5 ± 2.7	16.8 ± 0.8	31.9 ± 0.3	16.1 ± 0.9
16	♀	Homozygous ^huβ^IVSI-6, ^huβ^IVSI-6; ()	13.8 ± 0.2	8.8 ± 0.1	43.6 ± 1.0	49.3 ± 0.7	15.6 ± 0.2	31.7 ± 0.5	17.2 ± 0.2
			NS	P = 0.01	NS	P = 0.04	P = 0.02	NS	P = 0.03
16	♂	Wild-type ()	12.5 ± 0.6	7.6 ± 0.3	39.0 ± 1.4	51.5 ± 1.1	16.5 ± 0.2	31.9 ± 0.7	15.9 ± 0.8
16	♂	Homozygous ^huβ^IVSI-6, ^huβ^IVSI-6; ()	13.0 ± 0.3	8.6 ± 0.2	42.0 ± 1.1	49.1 ± 0.9	15.2 ± 0.1	31.0 ± 0.4	16.8 ± 0.2
			NS	P = 0.0002	P = 0.003	P = 0.003	P < 0.0001	P = 0.03	P = 0.03

Hematological values are expressed as means ± SD. Hemoglobin concentration (Hb), red blood cell count (RBC), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular Hb concentration (MCHC), red cell distribution width (RDW) are shown. indicates the number of analyzed mice. The   values of  Student’s -test, comparing each group of transgenic mice with wild-type mice controls, are also shown. NS corresponds to not statistically significant > 0.05.