Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

Table 4

Relative contents of β-globin transcripts.

Sample	Experiment	Peaks (%)
		115 bp	137 bp	153 bp	165 bp
Splicing site generating the transcript		(cryptic)	(cryptic)	+1 (normal)	+13 (cryptic)
ErPCs from normal donors	1	0	0	100	0
	2	0	0	100	0
	3	0	0	100	0
ErPCs from homozygous; β-IVSI-6/β-IVSI-6 patients	1	19.9	13.6	65.8	0.7
	2	16.1	13.7	69.2	1.0
	3	14.4	18.7	66.2	0.7
TG-β-IVSI-6 homozygous mouse #1	1	4.3	0.3	93.8	1.6
	2	7.6	0.1	88.6	3.7
TG-β-IVSI-6 homozygous mouse #2	1	6.5	0.4	91.1	2.0
	2	5.6	0.2	92.0	2.2
K562(β-IVSI-6) #1	1	21.6	2.2	74.6	1.6
	2	21.3	2.6	74.3	1.8
K562(β-IVSI-6) #2	1	22.3	3.0	73.6	1.1
MEL(hu β-globin gene)	1	0	0	100	0
MEL(hu β-globin gene), DMSO treated	1	0	0	100	0
MEL(hu β-IVSI-6 globin gene)	1	33.1	6.8	58.7	1.4
MEL(hu β-IVSI-6 globin gene), DMSO treated	1	38.5	5.8	54.5	1.2