Efficacy Study of Broken Rice Maltodextrin in In Vitro Wound Healing Assay
Table 1
(a) The percentage of wound closure of NIH 3T3 cells treated with RB maltodextrin DE 10–14 with or without the addition of various additives. (b) The percentage of wound closure of NIH 3T3 cells treated with 5%, 10%, or 20% RB maltodextrin DE 10–14 with or without the addition of 100 ppm various additives. (c) The percentage of wound closure of NIH 3T3 cells treated with RB maltodextrin DE 10–14, 15–19, or DE 20–24 with or without the addition of 100 ppm various additives.
(a)
Time, h
RB
RBCUR
RBHYD
RBAA
RBARG
RBLA
RBKA
MUL
CTRL
(i) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 10–14) with 50 ppm of various additives
0
0.00 ± 0.00
3
5.69 ± 3.05
9.23 ± 0.00
6.43 ± 1.84
6.16 ± 3.37
8.64 ± 3.92
5.81 ± 3.80
4.82 ± 4.43
0.00 ± 0.00
12.04 ± 0.53
6
15.72 ± 5.95
15.38 ± 0.00
18.21 ± 8.87
13.43 ± 4.89
18.06 ± 5.25
15.48 ± 3.92
10.66 ± 5.63
21.17 ± 1.72
9
23.85 ± 3.20
20.00 ± 0.00
28.21 ± 9.27
20.34 ± 0.60
24.61 ± 3.51
19.35 ± 6.64
23.35 ± 9.56
29.93 ± 4.63
12
40.38 ± 4.81
26.92 ± 0.00
35.36 ± 12.31
28.92 ± 0.87
30.63 ± 2.85
32.90 ± 4.06
31.98 ± 7.20
36.86 ± 1.47
24
100.00 ± 0.00
50.77 ± 0.00
50.36 ± 2.86
47.76 ± 3.33
46.86 ± 7.78
42.90 ± 0.54
41.12 ± 6.36
100.00 ± 0.00
(ii) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 10–14) with 100 ppm of various additives
0
0.00 ± 0.00
3
19.23 ± 1.65
15.79 ± 0.00
16.40 ± 0.17
14.81 ± 1.44
16.33 ± 1.79
17.74 ± 0.00
12.10 ± 0.62
0.00 ± 0.00
6
32.69 ± 0.70
29.82 ± 2.11
28.95 ± 4.96
23.33 ± 6.06
31.78 ± 0.92
28.09 ± 0.75
21.66 ± 0.00
9
48.73 ± 2.32
42.11 ± 6.88
36.36 ± 4.73
30.23 ± 1.52
38.32 ± 0.78
34.83 ± 0.00
29.26 ± 0.31
12
61.13 ± 1.39
52.97 ± 3.68
51.52 ± 2.72
47.78 ± 1.90
47.66 ± 0.75
44.94 ± 4.49
32.98 ± 0.56
24
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
(b)
Time, h
RB
RBCUR
RBHYD
RBAA
RBARG
RBLA
RBKA
MUL
CTRL
(i) The percentage of wound closure of NIH 3T3 cell treated with 5% RB maltodextrin (DE 10–14) with 100 ppm of various additives
0
0.00 ± 0.00
3
3.54 ± 0.76
2.51 ± 3.45
6.94 ± 3.51
7.39 ± 1.21
5.01 ± 0.60
7.27 ± 1.53
3.64 ± 2.49
2.04 ± 0.60
12.04 ± 0.53
6
12.96 ± 4.06
7.71 ± 1.06
13.87 ± 3.85
14.43 ± 4.04
11.22 ± 4.21
20.64 ± 5.11
10.75 ± 3.00
6.89 ± 3.19
21.17 ± 1.72
9
24.41 ± 5.17
21.68 ± 3.48
20.65 ± 5.77
20.62 ± 2.46
23.05 ± 7.83
32.27 ± 7.10
19.58 ± 2.64
8.42 ± 2.17
29.93 ± 4.63
12
33.50 ± 8.81
28.14 ± 1.33
27.90 ± 8.70
26.80 ± 6.94
28.86 ± 9.24
44.19 ± 4.17
27.90 ± 9.47
10.20 ± 0.57
36.86 ± 1.47
24
57.91 ± 5.67
55.56 ± 10.34
53.71 ± 2.41
48.97 ± 6.62
47.90 ± 3.76
46.51 ± 4.05
45.58 ± 8.33
10.71 ± 1.73
100.00 ± 0.00
(ii) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 10–14) with 100 ppm of various additives
3
19.23 ± 1.65
15.79 ± 0.00
16.40 ± 0.17
14.81 ± 1.44
16.33 ± 1.79
17.74 ± 0.00
12.10 ± 0.62
0.00 ± 0.00
6
32.69 ± 0.70
29.82 ± 2.11
28.95 ± 4.96
23.33 ± 6.06
31.78 ± 0.92
28.09 ± 0.75
21.66 ± 0.00
9
48.73 ± 2.32
42.11 ± 6.88
36.36 ± 4.73
30.23 ± 1.52
38.32 ± 0.78
34.83 ± 0.00
29.26 ± 0.31
12
61.13 ± 1.39
52.97 ± 3.68
51.52 ± 2.72
47.78 ± 1.90
47.66 ± 0.75
44.94 ± 4.49
32.98 ± 0.56
24
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
(iii) The percentage of wound closure of NIH 3T3 cell treated with 20% RB maltodextrin (DE 10–14) with 100 ppm of various additives
3
7.27 ± 0.00
11.24 ± 3.08
11.11 ± 0.00
10.61 ± 4.77
12.16 ± 2.66
6.93 ± 1.75
5.32 ± 4.22
0.00 ± 0.00
6
15.64 ± 3.88
16.43 ± 0.00
16.87 ± 3.18
22.07 ± 1.92
18.11 ± 0.24
20.50 ± 7.62
4.98 ± 2.81
9
20.73 ± 1.47
20.75 ± 0.00
23.46 ± 8.67
27.37 ± 2.97
22.43 ± 4.92
24.65 ± 4.94
11.63 ± 3.52
12
22.18 ± 0.84
27.38 ± 3.97
29.22 ± 9.62
33.80 ± 0.00
30.54 ± 3.48
36.01 ± 1.77
25.58 ± 2.42
24
72.73 ± 1.69
67.44 ± 0.90
66.26 ± 1.78
49.16 ± 3.76
44.59 ± 4.81
43.77 ± 3.35
34.55 ± 2.32
(c)
Time, h
RB
RBCUR
RBHYD
RBAA
RBARG
RBLA
RBKA
MUL
CTRL
(i) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 10–14) with 100 ppm of various additives
0
0.00 ± 0.00
3
19.23 ± 1.65
15.79 ± 0.00
16.40 ± 0.17
14.81 ± 1.44
16.33 ± 1.79
17.74 ± 0.00
12.10 ± 0.62
0.00 ± 0.00
12.04 ± 0.53
6
32.69 ± 0.70
29.82 ± 2.11
28.95 ± 4.96
23.33 ± 6.06
31.78 ± 0.92
28.09 ± 0.75
21.66 ± 0.00
21.17 ± 1.72
9
48.73 ± 2.32
42.11 ± 6.88
36.36 ± 4.73
30.23 ± 1.52
38.32 ± 0.78
34.83 ± 0.00
29.26 ± 0.31
29.93 ± 4.63
12
61.13 ± 1.39
52.97 ± 3.68
51.52 ± 2.72
47.78 ± 1.90
47.66 ± 0.75
44.94 ± 4.49
32.98 ± 0.56
36.86 ± 1.47
24
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
(ii) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 15–19) with 100 ppm of various additives
3
7.22 ± 1.54
10.96 ± 0.00
11.41 ± 0.00
9.29 ± 2.68
16.29 ± 3.23
7.02 ± 4.49
2.93 ± 0.62
0.00 ± 0.00
6
16.58 ± 2.07
18.36 ± 0.00
21.75 ± 3.75
25.70 ± 0.00
29.14 ± 4.80
18.73 ± 0.75
13.20 ± 0.00
9
22.99 ± 6.95
26.85 ± 9.73
33.42 ± 1.76
32.51 ± 1.52
39.71 ± 5.30
20.40 ± 0.75
17.30 ± 0.00
12
56.28 ± 3.40
50.41 ± 7.69
49.36 ± 5.38
45.82 ± 1.80
45.14 ± 8.24
31.10 ± 0.00
20.23 ± 0.00
24
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
100.00 ± 0.00
46.48 ± 0.00
28.15 ± 0.00
(iii) The percentage of wound closure of NIH 3T3 cell treated with 10% RB maltodextrin (DE 20–24) with 100 ppm of various additives
3
13.56 ± 3.34
9.30 ± 0.00
12.33 ± 0.00
17.11 ± 0.00
8.45 ± 0.00
14.10 ± 0.00
8.62 ± 0.00
0.00 ± 0.00
6
23.73 ± 0.00
23.26 ± 6.91
24.66 ± 0.00
27.63 ± 0.00
25.35 ± 0.00
26.92 ± 0.00
22.41 ± 0.00
9
33.90 ± 0.00
35.47 ± 4.24
41.10 ± 0.00
35.53 ± 0.00
25.35 ± 0.00
37.18 ± 0.00
41.38 ± 0.00
12
47.46 ± 0.00
47.67 ± 4.71
49.32 ± 0.00
44.74 ± 0.00
35.21 ± 0.00
46.15 ± 0.00
53.45 ± 0.00
24
66.67 ± 0.00
61.63 ± 3.46
65.75 ± 0.00
59.21 ± 0.00
47.89 ± 0.00
57.69 ± 0.00
63.79 ± 0.00
Each data was expressed as mean ± standard deviation of triplicate determinations. Mean values with different superscripts in the same column are significantly different p < 0.01. Mean values with different superscripts in the same row are significantly different p < 0.01. RB: broken rice maltodextrin; CUR: curcumin; HYD: hydroxyproline; AA: ascorbic acid; ARG: L-arginine; LA: lactic acid; KA: kojic acid; MUL: Multidex; CTRL: control.
