Interleukin-27 Protects Cardiomyocyte-Like H9c2 Cells against Metabolic Syndrome: Role of STAT3 Signaling

Figure 4

Effect of STAT3 inhibition on cell viability. (a) Cells were treated with stattic (dark gray bars) or stattic plus IL-27 (light gray bars) 4 h prior to treatment with glucose (25 mM, G), NaCl (250 mM, S), or cholesterol (300 μg mL⁻¹, C), either alone or in different combinations, for 4, 8, 24, and 48 h. Lactate dehydrogenase (LDH) was determined as a marker of cell injury. (b) Cells were cultured for 48 h in the presence of the indicated agents and viability was measured in an MTT assay. of experiments performed in each time-point of group. versus the corresponding control groups (stattic alone or stattic + IL-27); versus G; versus S; versus C; versus GS; versus GC; versus SC.

(a)

(b)