Interleukin-27 Protects Cardiomyocyte-Like H9c2 Cells against Metabolic Syndrome: Role of STAT3 Signaling

Figure 5

Effect of stattic on STAT3 activity and cytochrome c release. (a) Changes in STAT3 activity after 48 h of treatment with stattic, stattic + GSC, stattic + IL-27, or stattic + IL-27 + GSC. Note that stattic enhanced the high glucose, sodium, and cholesterol (GSC) mediated reduction in STAT3 activity and abrogated the STAT3-enhancing effects of IL-27. (b) Stattic increased LDH release in GSC-treated cells in the presence or absence of IL-27. (c) Apoptosis was evaluated by measuring cytochrome c (Cyto c) release. Western blots showing the expression of cytochrome c in three representative cell cultures. Equal amounts of protein (20 μg per lane) were loaded. The bar graph shows changes in the expression of cytochrome c in treated cells (after being normalized against actin). of experiments performed in each group. AU, arbitrary units. versus the stattic group; versus the stattic + IL-27 group.

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