Precursor Amino Acids Inhibit Polymyxin E Biosynthesis in Paenibacillus polymyxa, Probably by Affecting the Expression of Polymyxin E Biosynthesis-Associated Genes

Table 1

Sequences of primer pairs for PCR amplification of genes involved in polymyxin E biosynthesis.

Genes	Nucleotide sequences (5′-3′)		Product sizes (bp)
	Forward primers	Reverse primers
pmxA	CCGCCCATTATGACAACCGT	GCTGGCGAATTGAACGATCC	1238
pmxB	ATGAAATCTTTGTTTGAAAA	CCAGGACGTACACCCTCAAC	1878
pmxC	AAAGGCTGGATGATCGTCGG	AGAAAGCCGGTGGGCAATAA	1547
pmxD	ATGAAAAAGGGCGGATGGCT	CTAGCCGTACAGCCGGGCGT	1734
pmxE	GCAAAATCCAACGCAGTGGT	ACGTTGAGAATGCGGTGTCT	1135
spo0A	ATGAAAATATACGCGATTGA	TTACGCCTGTGTTGCCACTT	699
abrB	TCAGTTTGTCACAGGTGTTG	ATGATGAAATCCACTGGATA	255
ectB	ATGAACACATTCGAAACACT	TACGCCCAGCACGTTCAATA	1145
sfp	TTGCAAAAAATTGAGGTATT	TCAGGACACCTTATTCTCAA	804