The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides

Figure 4

Macropinocytosis is involved in the cell-penetration pathway of PTD-J-DsRed. Huh-7 cells were pretreated with filipin (5 μg/mL), EIPA (100 μM), or cytochalasin D (10 μM) for 1 hour before the treatment of the PTD-J-DsRed recombinant protein (40 μg/mL) for 2 hours. The fluorescence values of 100 μL cleared lysate samples were measured (three experiments were performed and the values of filipin, cytochalasin D, and EIPA data relative to the control are 0.0257, 0.000812, and 0.000165, resp.).