Research Article
Salivary Desmoglein Enzyme-Linked Immunosorbent Assay for Diagnosis of Pemphigus Vulgaris: A Noninvasive Alternative Test to Serum Assessment
Table 1
Salivary anti-Dsg 1 and anti-Dsg 3 ELISA tests main diagnostic outcomes using three different cut-off values.
| | Salivary anti-Dsg 1 ELISA | Salivary anti-Dsg 3 ELISA |
| Cut-off (RU/mL) | 5.4 | 7.7 | 20 | 8.9 | 13.4 | 20 | True positive | 48 | 45 | 31 | 69 | 67 | 63 | True negative | 174 | 178 | 178 | 163 | 176 | 178 | False positive | 6 | 2 | 2 | 17 | 4 | 2 | False negative | 38 | 41 | 55 | 17 | 19 | 23 | Sensitivity | 55.8% | 52.3% | 36.1% | 80.2% | 77.9% | 73.3% | Specificity | 96.7% | 98.9% | 98.9% | 90.6% | 97.8% | 98.9% | Positive predictive values | 0.89 | 0.96 | 0.94 | 0.80 | 0.94 | 0.97 | Negative predictive values | 0.82 | 0.81 | 0.76 | 0.91 | 0.90 | 0.89 | Test accuracy | 0.83 | 0.84 | 0.79 | 0.87 | 0.91 | 0.91 | Positive likelihood ratio (+LR) | 16.7 | 47.1 | 32.4 | 8.5 | 35.1 | 65.9 | Negative likelihood ratio (−LR) | 0.5 | 0.5 | 0.6 | 0.2 | 0.2 | 0.3 | Diagnostic odds ratio | 36.6 | 97.7 | 50.2 | 38.9 | 155.2 | 243.8 | Cohen’s kappa coefficient | 0.82 | 0.83 | 0.78 | 0.86 | 0.91 | 0.90 |
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