BioMed Research International / 2015 / Article / Fig 3

Research Article

Protective Effects of Cilastatin against Vancomycin-Induced Nephrotoxicity

Figure 3

Effect of cilastatin on vancomycin-induced early and late apoptosis. Vancomycin-induced early and late-apoptotic cell death in proximal tubular epithelial cells and the effect of cilastatin were determined by flow cytometry with annexin V/propidium iodide assay after 24 hours of treatments. (a) Representative scatter plots of propidium iodide ( axis) versus annexin V ( axis). The lower right quadrants represent the early-apoptotic cells (annexin V-positive/propidium iodide-negative) and the upper right quadrants represent the late-apoptotic cells (annexin V-positive/propidium iodide-positive). (b) Quantification of early-apoptotic cells in all conditions (lower right quadrants). (c) Quantification of late-apoptotic cells in all conditions (upper right quadrants). Results are expressed as % of total cells quantified. Data are represented as the mean SEM of at least three separate experiments. versus control and control plus cilastatin, versus the same data without cilastatin. IP, propidium iodide. Anex V, annexin V.