Cooperative Regulation of Substrate Stiffness and Extracellular Matrix Proteins in Skin Wound Healing of Axolotls

Table 1

Effect of substrate stiffness and extracellular matrix proteins on wound closure in skin explants culture.

	Stiffness (kPa)	Number of skin explants	Complete wound closure	Partial wound closure	No wound closure
Collagen; (20 μg/cm^2)	0.2	6	0	4	2
	2	6	5	1	0
	12	6	1	5	0
	50	6	2	4	0
Tenascin; (2 μg/cm^2)	0.2	6	0	0	6
	2	6	0	0	6
	12	6	0	0	6
	50	6	0	0	6
Fibronectin; (2 μg/cm^2)	0.2	6	0	0	6
	2	6	0	0	6
	12	6	0	0	6
	50	6	0	2	4
Laminin; (2 μg/cm^2)	0.2	6	0	0	6
	2	6	0	0	6
	12	6	0	0	6
	50	6	0	0	6

Skin explants were sliced into 4 × 4 mm² and punched 2 mm hole in the center.
^*Wound closure was determined and recorded after 15 h culture.