Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts

Figure 4

MG63-GFP gene expression. Fold change in relative gene expression of MG63-GFP cells normalized to RPLP at day 0 quantified by real-time PCR. Bars show the mean ± SEM of 3 different experiments performed in triplicate per group ( per group with the exception of (a, c, e, g, i, and k) in DM, where ). Mean ± SEM; D’Agostino-Pearson omnibus normality test was used followed by one-way ANOVA; ; ; . (a) Runx2 on days 2 and 7, (b) Runx2 on days 14 and 21, (c) Sox9 on days 2 and 7, (d) Sox9 on days 14 and 21, (e) ALP on days 2 and 7, (f) ALP on days 14 and 21, (g) Type 1 Col on days 2 and 7, (h) Type 1 Col on days 14 and 21, (i) OC on days 2 and 7, and (j) OC on days 14 and 21.

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