Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts

Figure 9

Alizarin Red S quantification. Bounded ARS is related to total DNA content and expressed in nmol/μg DNA. Bars show the mean ± SEM of 3 different experiments performed in triplicate per group with the exception of (b) where 2 different experiments were performed; one-way ANOVA was used to test for statistical significance. (a) ARS content in hMSC culture on day 28 ( per group). (b) ARS content in MG63-GFP cell culture on day 28 (DM ± IL-1β,   per group; CM ± IL-1β,   per group). Notice: -axis is gapped.

(a)

(b)