BioMed Research International / 2015 / Article / Fig 1

Research Article

Redesigning Protein Cavities as a Strategy for Increasing Affinity in Protein-Protein Interaction: Interferon-γ Receptor 1 as a Model

Figure 1

Nonreducing 12.5% SDS-PAGE gel of selected monomeric refolded recombinant His-tagged IFNγR1 variants. Proteins were extracted from inclusion bodies by 8 M urea, further purified on Ni-NTA agarose, and dialyzed, and monomeric fraction was separated on gel filtration column (see above). IFNγR1 with C-terminal His-Tag migrates at a molecular mass of 23 kDa when analyzed on nonreducing SDS-PAGE gel.

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