Gab1 Is Modulated by Chronic Hypoxia in Children with Cyanotic Congenital Heart Defect and Its Overexpression Reduces Apoptosis in Rat Neonatal Cardiomyocytes

Figure 4

Gab1 protein expression in rat cardiomyocytes following 24 h of hypoxic treatment. Cells were treated with hypoxic conditions (H) by incubating in a chamber with 5% CO₂/0.2% O₂ or normoxia (N) by leaving cells in normal CO2 incubator. After 24 h cells were lysed and western blot analysis performed using GAB1 (a), SHP2 (b), and p85 (c) antibodies. All results were normalized to GAPDH levels. Data are mean ± SEM. ().

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