Gab1 Is Modulated by Chronic Hypoxia in Children with Cyanotic Congenital Heart Defect and Its Overexpression Reduces Apoptosis in Rat Neonatal Cardiomyocytes
(a) Protein expression level of Gab1, p85, and SHP2 in infected rat neonatal cardiomyocytes. Cardiac myocytes were infected with Ad-β-Gal (50 MOI) and Ad-Gab1-WT (10 MOI), for 24 hours. GAPDH was used as a loading control. (b) Apoptosis quantification of infected rat neonatal cardiomyocytes subjected to normoxia and hypoxia. Cardiac myocytes were infected with Ad-β-galactosidase (Ad-β-Gal) and Ad-Gab1-WT, for 24 hours. Data are mean ± SEM. . (c) Representative pictures of the TUNEL assay performed on rat neonatal cardiomyocytes infected by Ad-β-Gal or Ad-Gab1-WT and subjected to normoxia or hypoxia. Arrows show apoptotic nuclei (green fluorescence). Cell nuclei were stained by DAPI (blue fluorescence). Magnification: ×400.
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