Immunomodulatory Effects of the Agaricus blazei Murrill-Based Mushroom Extract AndoSan in Patients with Multiple Myeloma Undergoing High Dose Chemotherapy and Autologous Stem Cell Transplantation: A Randomized, Double Blinded Clinical Study
Table 3
T-lymphocyte subsets and dendritic cell subsets.
Agaricus
( = 16)
Placebo
(=17)
value
Mean
Range
Mean
Range
% of T-lymphocytes
CD3+ T cells
86.2
(58.9–94.2)
86.7
(71.7–95.3)
n.s.
CD4+ T cells
50.8
(20.0–80.4)
51.8
(7.10–73.6)
n.s.
CD8+ T cells
40.4
(10.9–67.9)
39.6
(15.7–83.3)
n.s.
% of CD4+ T cells
Naive (CD45RA+/CD27+)
24.7
(5.5–64.0)
29
(8.8–51.4)
n.s.
Central memory (CD45RA−/CD27+)
40.8
(20.3–72.3)
46.7
(30.0–73.5)
n.s.
Effector memory (CD45RA−/CD27−)
19.2
(3.1–37.2)
21.8
(5.5–38.8)
n.s
Terminally differentiated memory (CD45RA+/CD27−)
6
(0.3–38.5)
3.1
(0.2–9.4)
n.s
T reg = (CD4+/CD127d+/Cd25+)
11.8
(4.5–18.2)
9
(4.0–17.8)
= 0.04
HLA-DR+ CD4+
29.6
(13.4–55.7)
26.6
(7.9–55.0)
n.s
% of CD8+ T cells
Naive (CD45RA+/CD27+)
27.8
(2.4–69.9)
31.4
(5.9–62.5)
n.s
Central memory (CD45RA−/CD27+)
17.3
(1.5–32.8)
20.1
(3.2–43.5)
n.s.
Effector memory (CD45RA−/CD27−)
22.9
(4.4–51.7)
16.8
(4.6–49.3)
n.s.
Terminally differentiated memory (CD45RA+/CD27−)
32.8
(1.4–55.7)
31.7
(5.4–79.0)
n.s
HLA-DR+ CD8+
39.1
(7.5–71.6)
36.2
(5.9–69.3)
n.s
Others
%NK cells (CD2 or CD7+ CD3−)
8.4
(0.9–35.8)
6.7
(1.7–26.7)
n.s.
% CD56b+
9.3
(0.7–35.5)
10.7
(0.1–32.7)
n.s.
% CD56b+ CD16+
70.6
(42.2–94.0)
69.4
(39.9–95.5)
n.s.
%CD56−CD16+
6.4
(0.1–34.1)
2.6
(0.1–15.3)
n.s.
%CD94+
65.5
(25.9–96.4)
60.8
(22.1–84.7)
n.s.
% of all cells except for CD14+ monocytes and CD19+ cells
BDCA1 (CD1c+)
0.6
(1.0–1.3)
0.6
(0.1–2.4)
n.s.
BDCA2 (CD303)
1.1
(0.2–2.3)
0.7
(0.1–1.4)
= 0.04
BDCA3 (CD141)
0.1
(0.04–0.4)
0.1
(0.03–0.3)
n.s.
The respective cell populations are given as frequencies of the cellpopulation to which it is a subset: T-cells and NK-cells as percentage of total lymphocytes; the major T-cell subsets (including CD4 positive, CD8 positive, and CD4/CD8 double negative or double positive); and NK cell subsets (including CD94 positive, CD94 positive, CD56 bright positive, CD56 positive, Cd16 positive, and CD16 positive) as percentages of total T-cells and NK-cells, respectively: naive, central memory, effector memory, and terminally differentiated memory T-cells as well as CD4 positive T regulatory T-cells of CD4 and CD8 positive T-cells, respectively. The dendritic cell populations are determined within total cells excluding the CD14 positive and CD19 positive cells.
